Biosynthesis of 9-Methylstreptimidone Involves a New Decarboxylative Step for Polyketide Terminal Diene Formation

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• 作者：Bo Wang ; Yongxiang Song ; Minghe Luo ; Qi Chen ; Junying Ma ; Hongbo Huang ; Jianhua Ju
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：March 15, 2013
• 年：2013
• 卷：15
• 期：6
• 页码：1278-1281
• 全文大小：217K
• 年卷期：v.15,no.6(March 15, 2013)
• ISSN：1523-7052

文摘

9-Methylstreptimidone is a glutarimide antibiotic showing antiviral, antifungal, and antitumor activities. Genome scanning, bioinformatics analysis, and gene inactivation experiments reveal a gene cluster responsible for the biosynthesis of 9-methylstreptimidone in Streptomyces himastatinicus. The unveiled machinery features both acyltransferase- and thioesterase-less iterative use of module 5 as well as a branching module for glutarimide generation. Impressively, inactivation of smdK leads to a new carboxylate analogue unveiling a new mechanism for polyketide terminal diene formation.