Primary Cell Culture of Fresh Water Hyriopsis cumingii Mantle/Pearl Sac Tissues and Its Effect on Calcium Carbonate Mineralization

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• 作者：Dongni Ren ; Olga Albert ; Minghui Sun ; Werner E. G. M眉ller ; Qingling Feng
• 刊名：Crystal Growth & Design
• 出版年：2014
• 出版时间：March 5, 2014
• 年：2014
• 卷：14
• 期：3
• 页码：1149-1157
• 全文大小：577K
• 年卷期：v.14,no.3(March 5, 2014)
• ISSN：1528-7505

文摘

Primary cell cultures of the fresh water Hyriopsis cumingii mantle and pearl sac tissues were produced in this study, and the influence of the tissue, cells, and secreted protein on calcium carbonate crystal nucleation and growth was studied. The study contributes to a further understanding of the influence of organic matrices on CaCO₃ crystal formation. This research started from the protein level to the tissue/cell level, which is crucial for understanding the inorganic deposition process. The new data also add relevant theoretical approaches to an overall understanding of biomineralization processes. In the experimental groups with mantle or pearl sac tissue, the growth patterns of aragonite were similar: both started from a round disk-shaped amorphous calcium carbonate (ACC) and then turned to flowerlike aragonite aggregate. The whole crystal growing process was recorded by transmitted light microscopy. In the control group, without any tissue, there was no ACC found nor crystal phase transformation; it was pure calcite, and the crystal size enlarged as the culture time increased.