Complexin-1 Enhances the On-Rate of Vesicle Docking via Simultaneous SNARE and Membrane Interactions

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• 作者：Jiajie Diao ; Daniel J. Cipriano ; Minglei Zhao ; Yunxiang Zhang ; Sachi Shah ; Mark S. Padolina ; Richard A. Pfuetzner ; Axel T. Brunger
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：October 16, 2013
• 年：2013
• 卷：135
• 期：41
• 页码：15274-15277
• 全文大小：243K
• 年卷期：v.135,no.41(October 16, 2013)
• ISSN：1520-5126

文摘

In synaptic terminals, complexin is thought to have inhibitory and activating roles for spontaneous 鈥渕ini鈥?release and evoked synchronized neurotransmitter release, respectively. We used single vesicle鈥搗esicle microscopy imaging to study the effect of complexin-1 on the on-rate of docking between vesicles that mimic synaptic vesicles and the plasma membrane. We found that complexin-1 enhances the on-rate of docking of synaptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasma membrane-mimicking vesicles containing full-length syntaxin-1A and SNAP-25A. This effect requires the C-terminal domain of complexin-1, which binds to the membrane, the presence of PS in the membrane, and the core region of complexin-1, which binds to the SNARE complex.