Flow Cytometry: A New Method To Investigate the Properties of Water-in-Oil-in-Water Emulsions

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• 作者：Mingtan Hai ; Kalia Bernath ; Dan Tawfik ; and Shlomo Magdassi
• 刊名：Langmuir
• 出版年：2004
• 出版时间：March 16, 2004
• 年：2004
• 卷：20
• 期：6
• 页码：2081 - 2085
• 全文大小：131K
• 年卷期：v.20,no.6(March 16, 2004)
• ISSN：1520-5827

文摘

We present a new and facile method to evaluate w/o/w emulsions containing fluorescent markers by flowcytometry. Flow cytometry allows simultaneous measurement of w/o/w emulsion droplets "marked" witha fluorescent marker or "blank" without the need for complicated sample preparation. The yield of preparationof the w/o/w emulsion and the release rate of the fluorescent marker FITC-BSA were investigated by thisnew method. The release fraction (after 24 h) of FITC-BSA from the w/o/w emulsion decreased withincreasing concentration of FITC-BSA inside the internal phase, just like the release fraction of NaClas marker from the w/o/w emulsion. Flow cytometry results show that the yield and release behavior inw/o/w emulsions are in agreement with results reported by more complicated methods.