Highly Stable, Water-Soluble, Intrinsic Fluorescent Hybrid Scaffolds for Imaging and Biosensing

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• 作者：Ranjith Krishna Pai ; Mircea Cotlet
• 刊名：Journal of Physical Chemistry C
• 出版年：2011
• 出版时间：February 10, 2011
• 年：2011
• 卷：115
• 期：5
• 页码：1674-1681
• 全文大小：944K
• 年卷期：v.115,no.5(February 10, 2011)
• ISSN：1932-7455

文摘

A synthetic method is proposed for the preparation of micrometer-sized hybrid materials in the form of highly stable, water-soluble, porous, and intrinsic fluorescent vaterites. This is an easy, cost-effective, and polymer-free synthetic method, that is, free of any supplementary complex synthetic or natural macromolecular stabilizers. This method uses a double decomposition reaction to introduce fluorescence as an intrinsic property into the vaterite scaffold, through either organic dyes or dihydrolipoic acid coated core/shell CdSe/ZnS quantum dots. The resulting hybrid scaffold has excellent brightness, photostability, thermal stability, and pH stability. Combined with a large loading surface offered by the vaterite scaffold and the ease of chemical functionalization provided by the water-soluble quantum dots, the obtained hybrid scaffolds show promise in biological applications. Fluorescence imaging and fluorescence-resonance-energy-transfer-based sensing of proteins based on these hybrid materials is illustrated with these hybrid materials at the single-particle level.