Effect of Dietary Conjugated Linoleic Acid on Lipid Peroxidation and Histological Change in Rat Liver Tissues

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• 作者：Masao Yamasaki ; Keiko Mansho ; Hiroko Mishima ; Genki Kimura ; Masafumi Sasaki ; Masaaki Kasai ; Hirofumi Tachibana ; and Koji Yamada
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2000
• 出版时间：December 2000
• 年：2000
• 卷：48
• 期：12
• 页码：6367 - 6371
• 全文大小：161K
• 年卷期：v.48,no.12(December 2000)
• ISSN：1520-5118

文摘

The effect of dietary conjugated linoleic acid (CLA) on hepatic lipid parameters in Sprague-Dawleyrats was examined. When rats were fed a diet containing CLA at 0 (control), 1, or 2% of the weightof the amount of food given for 3 weeks, the liver weight exhibited a slight increase in the CLA-fedgroups, although the difference was not significant. Lipid accumulation in the hepatocytes of CLA-fed rats was also demonstrated by electron microscopic observation. In addition, the liverthiobarbituric acid reactive substances levels were significantly higher in the 2 wt % CLA groupthan in the other two dietary groups, and the levels of phosphatidylcholine hydroperoxide werehigher in CLA-fed groups when compared to that of the control group. On the other hand, the serumlipid peroxide levels were comparable among all three dietary groups. Levels of triglycerides in thewhite adipose tissue (WAT) and serum nonesterified fatty acid (NEFA) were reduced in a CLA-dose-dependent manner. CLA was shown to accumulate in the WAT much more than in the serumor liver. These results suggest that CLA accelerates the decomposition of storage lipids in WATand the clearance of serum NEFA levels, resulting in lipid peroxidation and a morphological changein the liver.Keywords: Conjugated linoleic acid; liver histology; phosphatidylcholine hydroperoxide; thiobarbituric acid reactive substances