Molecular Basis for Stellatic Acid Biosynthesis: A Genome Mining Approach for Discovery of Sesterterpene Synthases

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• 作者：Yudai Matsuda ; Takaaki Mitsuhashi ; Zhiyang Quan ; Ikuro Abe
• 刊名：Organic Letters
• 出版年：2015
• 出版时间：September 18, 2015
• 年：2015
• 卷：17
• 期：18
• 页码：4644-4647
• 全文大小：269K
• ISSN：1523-7052

文摘

The search for a new sesterterpene synthase in the genome of Emericella variecolor, which reportedly produces diverse sesterterpenoids, is described. One gene product (a chimeric protein with prenyltransferase and terpene cyclase domains) led to the synthesis of a novel tricyclic sesterterpene, stellata-2,6,19-triene (1), from DMAPP and IPP, and the hydrocarbon was further transformed into stellatic acid (2) by cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene.