Enhancement of Activity of Lipase-Displaying Yeast Cells and Their Application to Optical Resolution of (R,S)-1-Benzyloxy-3-Chloro-2-Propyl Monosuccinate
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- 作者：Yurie Nakamura ; Takeshi Matsumoto ; Fumiki Nomoto ; Mitsuyoshi Ueda ; Hideki Fukuda ; Akihiko Kondo
- 刊名：Biotechnology Progress
- 出版年：2006
- 出版时间：August 2006
- 年：2006
- 卷：22
- 期：4
- 页码：998 - 1002
- 全文大小：81K
- 年卷期：v.22,no.4(August 2006)
- ISSN：1520-6033
文摘
Rhizopus oryzae lipase (ROL) was displayed on the cell surface of Saccharomyces cerevisiaevia the Flo1 N-terminal region (1100 amino acids), which corresponds to a flocculation functionaldomain. The activity of lipase-displaying yeast whole-cell biocatalysts was enhanced 7.3-foldby incubation of the yeast cells at 20 
C in distilled water for 8 days after 8 day cultivation. Theamount of lipase molecules present in cell wall and intracellular fractions was found to beincreased 4.5- and 1.8-fold, respectively, by incubation, which proves that ROL molecules areexpressed during incubation. The ROL-displaying yeast whole-cell biocatalyst with enhancedactivity was successfully catalyzed by optical resolution of the pharmaceutical precursor (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate. Moreover, it showed stable activity through atleast eight reaction cycles. These results demonstrate that ROL-displaying yeast cells withenhanced activity by incubation in distilled water are very effective in industrial bioconversionprocesses.
C in distilled water for 8 days after 8 day cultivation. Theamount of lipase molecules present in cell wall and intracellular fractions was found to beincreased 4.5- and 1.8-fold, respectively, by incubation, which proves that ROL molecules areexpressed during incubation. The ROL-displaying yeast whole-cell biocatalyst with enhancedactivity was successfully catalyzed by optical resolution of the pharmaceutical precursor (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate. Moreover, it showed stable activity through atleast eight reaction cycles. These results demonstrate that ROL-displaying yeast cells withenhanced activity by incubation in distilled water are very effective in industrial bioconversionprocesses.