Destabilizing Domains Derived from the Human Estrogen Receptor

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• 作者：Yusuke Miyazaki ; Hiroshi Imoto ; Ling-chun Chen ; Thomas J. Wandless
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：March 7, 2012
• 年：2012
• 卷：134
• 期：9
• 页码：3942-3945
• 全文大小：222K
• 年卷期：v.134,no.9(March 7, 2012)
• ISSN：1520-5126

文摘

Methods to rapidly and reversibly perturb the functions of specific proteins are desirable tools for studies of complex biological processes. We have demonstrated an experimental strategy to regulate the intracellular concentration of any protein of interest by using an engineered destabilizing protein domain and a cell-permeable small molecule. Destabilizing domains have general utility to confer instability to a wide range of proteins including integral transmembrane proteins. This study reports a destabilizing domain system based on the ligand binding domain of the estrogen receptor that can be regulated by one of two synthetic ligands, CMP8 or 4-hydroxytamoxifen.