Widespread Occurrence of the Mycotoxin Fumonisin B2 in Wine
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- 作者：Jesper Mø ; lgaard Mogensen ; Thomas Ostenfeld Larsen ; Kristian Fog Nielsen
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：April 28, 2010
- 年：2010
- 卷：58
- 期：8
- 页码：4853-4857
- 全文大小：694K
- 年卷期：v.58,no.8(April 28, 2010)
- ISSN：1520-5118
文摘
Fumonisins are important mycotoxins because they are suspected to cause human and animal toxicoses by the consumption of contaminated corn-based food and feeds. However, with the discovery of fumonisin production in grapes by Aspergillus niger, wine may also be a fumonisin-containing commodity. In the present study, we have developed a simple and quantitative cation-exchange-based purification method for the subsequent isotope dilution liquid chromatography−tandem mass spectrometry (LC−MS/MS) determination of fumonisins in wine. A comparative study of seven different solid-phase extraction (SPE) columns showed that polymeric mixed-mode reversed-phase (RP) cation-exchange columns were superior to classic silica-based cation and mixed-mode cation-exchange columns. A total of 77 wine samples from 13 countries were subsequently tested, and surprisingly, 18 (23%) were found to contain fumonisin B2 in the range of 1−25 μg/L. These findings were further confirmed by immunoaffinity purification and re-analysis of the positive cation-exchanged extracts.