d-Amino Acid Detection in Peptides by MALDI-TOF-TOF

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• 作者：Emmanuelle Sachon ; Gilles Clodic ; Ccile Galanth ; Mohamed Amiche ; Cline Ollivaux ; Daniel Soyez ; Grard Bolbach
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：June 1, 2009
• 年：2009
• 卷：81
• 期：11
• 页码：4389-4396
• 全文大小：260K
• 年卷期：v.81,no.11(June 1, 2009)
• ISSN：1520-6882

文摘

Detection of a d-amino acid residue in natural peptides by mass spectrometry remains a challenging task, as this post-translational modification does not induce any change in molecular mass. To our knowledge, the present article is the first report using matrix-assisted laser desorption/ionization (MALDI) for the discrimination and the quantification of peptide isomers. In this work, we used synthetic hepta- and decapeptides of biological relevance and their isomers. All-l sequences and some isomers containing a d-residue in various positions were analyzed.