Immunosensing of Atrazine with Antibody-Functionalized Cu-MOF Conducting Thin Films

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• 作者：Sanjeev K. Bhardwaj ; Neha Bhardwaj ; Girish C. Mohanta ; Pawan Kumar ; Amit L. Sharma ; Ki-Hyun Kim ; Akash Deep
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2015
• 出版时间：December 2, 2015
• 年：2015
• 卷：7
• 期：47
• 页码：26124-26130
• 全文大小：445K
• ISSN：1944-8252

文摘

This work reports the assembly of thin films of a silica (SiO₂)-modified copper鈥搈etal organic framework, Cu₃(BTC)₂ [Cu₃(BTC)₂@SiO₂, BTC = benzene-1,3,5-tricarboxylic acid] on a conducting substrate of NH₂鈥揃DC [NH₂鈥揃DC = 2-aminobenzene-1,4-dicarboxylic acid] doped polyaniline (PANI). Assembled Cu₃(BTC)₂@SiO₂/BDC-PANI thin films displayed electrical conductivity in the range of 35 渭A. These thin films were conjugated with antiatrazine antibodies to create a novel immunosensing platform. Various structural and spectral characteristics of the synthesized material and its bioconjugate were investigated. The developed immunosensor was used for the conductometric sensing of atrazine. The detection of atrazine was achieved with a high sensor sensitivity (limit of detection = 0.01 nM) and specificity in the presence of diverse pesticides (e.g., endosulfan, parathion, paraoxon, malathion, and monochrotophos).