Novel Hexapeptide Interacts with Tubulin and Microtubules, Inhibits A尾 Fibrillation, and Shows Significant Neuroprotection
详细信息 查看全文
- 作者：Atanu Biswas ; Prashant Kurkute ; Suraiya Saleem ; Batakrishna Jana ; Saswat Mohapatra ; Prasenjit Mondal ; Anindyasundar Adak ; Subhajit Ghosh ; Abhijit Saha ; Debmalya Bhunia ; Subhash Chandra Biswas ; Surajit Ghosh
- 刊名：ACS Chemical Neuroscience
- 出版年：2015
- 出版时间：August 19, 2015
- 年：2015
- 卷：6
- 期：8
- 页码：1309-1316
- 全文大小：499K
- ISSN：1948-7193
文摘
Herein, we report a novel hexapeptide, derived from activity dependent neuroprotective protein (ADNP), that spontaneously self-assembles to form antiparallel 尾-sheet structure and produces nanovesicles under physiological conditions. This peptide not only strongly binds with 尾-tubulin in the taxol binding site but also binds with the microtubule lattice in vitro as well as in intracellular microtubule networks. Interestingly, it shows inhibition of amyloid fibril formation upon co-incubation with A尾 peptide following an interesting mechanistic pathway and excellent neuroprotection in PC12 cells treated with anti-nerve growth factor (NGF). The potential of this hexapeptide opens up a new paradigm in design and development of novel therapeutics for AD.