Analysis of Fumonisin B1 in Fusarium proliferatum-Infected Asparagus Spears and Garlic Bulbs from Germany by Liquid Chromatography-Electrospray Ionization Mass Spectrometry

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• 作者：Walburga Seefelder ; Monika Gossmann ; and Hans-Ulrich Humpf
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：May 8, 2002
• 年：2002
• 卷：50
• 期：10
• 页码：2778 - 2781
• 全文大小：55K
• 年卷期：v.50,no.10(May 8, 2002)
• ISSN：1520-5118

文摘

Fusarium proliferatum is one of a group of fungal species that produce fumonisins and is consideredto be a pathogen of many economically important plants. The occurrence of fumonisin B₁ (FB₁) in F.proliferatum-infected asparagus spears from Germany was investigated using a liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method with isotopically labeledfumonisin FB₁-d₆ as internal standard. FB₁ was detected in 9 of the 10 samples in amounts rangingfrom 36.4 to 4513.7 ng/g (based on dry weight). Furthermore, the capability of producing FB₁ by thefungus in garlic bulbs was investigated. Therefore, garlic was cultured in F. proliferatum-contaminatedsoil, and the bulbs were screened for infection with F. proliferatum and for the occurrence of fumonisinsby LC-MS. F. proliferatum was detectable in the garlic tissue, and all samples contained FB₁ (26.0-94.6 ng/g). This is the first report of the natural occurrence of FB₁ in German asparagus spears, andthese findings suggest a potential for natural contamination of garlic bulbs with fumonisins.Keywords: Fumonisins; Fusarium proliferatum; toxins; asparagus; garlic; liquid chromatography-electrospray ionization mass spectrometry