Stable Polymethacrylate Nanocapsules from Ultraviolet Light-Induced Template Radical Polymerization of Unilamellar Liposomes

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• 作者：Joana Filipa Pereira da Silva Gomes ; Andreas F.-P. Sonnen ; Astrid Kronenberger ; Jü ; rgen Fritz ; Manuel Á ; lvaro Neto Coelho ; Didier Fournier ; Clara Fournier-Nö ; el ; Monique Mauzac ; Mathias W
• 刊名：Langmuir
• 出版年：2006
• 出版时间：August 29, 2006
• 年：2006
• 卷：22
• 期：18
• 页码：7755 - 7759
• 全文大小：402K
• 年卷期：v.22,no.18(August 29, 2006)
• ISSN：1520-5827

文摘

We employed UV-induced template polymerization to create hollow nanometer-sized polymer capsules. Homogeneous,unilamellar liposomes served as a two-dimensional template for the cross-linking of either butyl methacrylate orhydroxyethyl methacrylate with the bifunctional ethyleneglycol dimethacrylate. Different molar ratios of lipid/hydrophobicmonomer/bifunctional monomer/photoinitiator were tested and dynamic light scattering revealed negligible changesof size at a defined molar ratio of 2/1/10/20, respectively. Cryo-transmission electron microscopy provided clearevidence that incorporation of the methacrylate monomers into and polymerization in the hydrophobic bilayer phasedoes not disrupt vesicle integrity. Moreover, after solubilization of the lipids, the polymethacrylate nanocapsules werestable at conditions needed for negative staining and could be visualized by atomic force microscopy. In contrast toprevious findings, the nanocapsule size and shape did not change considerably after removal of the template phase,and the size distribution remained strictly monomodal. The employed method is not only an advance to fortifyliposomes, but the nanocapsules themselves can be functionalized.