Discovery of Differentially Expressed Genes Related to Histological Subtype of Hepatocellular Carcinoma

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• 作者：Dongkwon Lee ; Sang Wook Choi ; Myengsoo Kim ; Jin Hyun Park ; Moonkyu Kim ; Jungchul Kim ; and In-Beum Lee
• 刊名：Biotechnology Progress
• 出版年：2003
• 出版时间：June 2003
• 年：2003
• 卷：19
• 期：3
• 页码：1011 - 1015
• 全文大小：119K
• 年卷期：v.19,no.3(June 2003)
• ISSN：1520-6033

文摘

Hepatocellular carcinoma (HCC) is one of the most common human malignancies inthe world. To identify the histological subtype-specific genes of HCC, we analyzed thegene expression profile of 10 HCC patients by means of cDNA microarray. We proposeda systematic approach for determining the discriminatory genes and revealing thebiological phenomena of HCC with cDNA microarray data. First, normalization ofcDNA microarray data was performed to reduce or minimize systematic variations.On the basis of the suitably normalized data, we identified specific genes involved inhistological subtype of HCC. Two classification methods, Fisher's discriminant analysis(FDA) and support vector machine (SVM), were used to evaluate the reliability of theselected genes and discriminate the histological subtypes of HCC. This study mayprovide a clue for the needs of different chemotherapy and the reason for heterogeneityof the clinical responses according to histological subtypes.