Diagnostic Microarray for Influenza B Viruses

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• 作者：Daniela M. Dankbar ; Erica D. Dawson ; Martin Mehlmann ; Chad L. Moore ; James A. Smagala ; Michael W. Shaw ; Nancy J. Cox ; Robert D. Kuchta ; Kathy L. Rowlen
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：March 1, 2007
• 年：2007
• 卷：79
• 期：5
• 页码：2084 - 2090
• 全文大小：385K
• 年卷期：v.79,no.5(March 1, 2007)
• ISSN：1520-6882

文摘

The importance of global influenza surveillance usingsimple and rapid diagnostics has been frequently highlighted. For influenza type B, the need exists for discrimination between the two currently circulating major lineages, represented by virus strains B/Victoria/2/87 andB/Yamagata/16/88, as only one of these lineages isrepresented in seasonal influenza vaccines. Here, thedevelopment and characterization of a low-density DNAmicroarray (designated BChip) designed to detect andidentify the two influenza B lineages is presented. Theassay involved multiplex nucleic acid amplification andmicroarray hybridization of viral RNA. Detection andlineage identification was achieved in less than 8 h. In astudy of 62 influenza B virus samples from 19 countries,dating from 1945 to 2005, as well as 5 negative controlsamples, the assay exhibited 97% sensitivity and 100%specificity. Furthermore, application of a trained artificialneural network to the pattern of relative fluorescencesignals resulted in correct lineage assignment for 94% of50 applicable influenza B viruses, with no false assignments.