文摘
This study was designed to investigate the cancer preventive activities of wheat bran (WB) oil. Westudied the colon cancer preventive effects of WB oil and its subfractions in the Apcmin/+ mouse model,a recognized mouse model for human colorectal cancer, and used human colon cancer cell lines(HCT-116 and HT-29) to identify possible active fractions in WB oil. Our results showed that the oilfraction of WB was more active than the water fraction against the growth of human colon cancercell lines and that 2% WB oil significantly inhibited the overall tumorigenesis by 35.7% (p < 0.0001)in the Apcmin/+ mouse model. The WB oil was further fractioned into nonpolar lipids and phytochemicalsand the phytochemical fraction was fractionated into phytosterols and phytosterol ferulates, 5-alk(en)ylresorcinols, and unidentified constituents by normal phase silica gel column chromatography.Results on cell culture showed that the phytochemical fraction had a higher inhibitory effect on HCT-116 human colon cancer cells than that of WB oil, whereas the nonpolar lipid fraction had less growthinhibitory effectiveness. However, neither fractions showed a stronger inhibition than WB oil in theApcmin/+ mouse model. The current results demonstrate, for the first time, the intestinal cancerpreventive activity of WB oil. The active ingredients, however, remain to be identified.