Complexation of Amyloid Fibrils with Charged Conjugated Polymers

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• 作者：Dhiman Ghosh ; Paulami Dutta ; Chanchal Chakraborty ; Pradeep K. Singh ; A. Anoop ; Narendra Nath Jha ; Reeba S. Jacob ; Mrityunjoy Mondal ; Shruti Mankar ; Subhadeep Das ; Sudip Malik ; Samir K. Maji
• 刊名：Langmuir
• 出版年：2014
• 出版时间：April 8, 2014
• 年：2014
• 卷：30
• 期：13
• 页码：3775-3786
• 全文大小：691K
• 年卷期：v.30,no.13(April 8, 2014)
• ISSN：1520-5827

文摘

It has been suggested that conjugated charged polymers are amyloid imaging agents and promising therapeutic candidates for neurological disorders. However, very less is known about their efficacy in modulating the amyloid aggregation pathway. Here, we studied the modulation of Parkinson鈥檚 disease associated 伪-synuclein (AS) amyloid assembly kinetics using conjugated polyfluorene polymers (PF, cationic; PFS, anionic). We also explored the complexation of these charged polymers with the various AS aggregated species including amyloid fibrils and oligomers using multidisciplinary biophysical techniques. Our data suggests that both polymers irrespective of their different charges in the side chains increase the fibrilization kinetics of AS and also remarkably change the morphology of the resultant amyloid fibrils. Both polymers were incorporated/aligned onto the AS amyloid fibrils as evident from electron microscopy (EM) and atomic force microscopy (AFM), and the resultant complexes were structurally distinct from their pristine form of both polymers and AS supported by FTIR study. Additionally, we observed that the mechanism of interactions between the polymers with different species of AS aggregates were markedly different.