Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia)
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- 作者：Aina Ramsay ; Chris Drake ; Anja Grosse Brinkhaus ; Marion Girard ; Giuseppe Copani ; Frigga Dohme-Meier ; Giuseppe Bee ; Vincent Niderkorn ; Irene Mueller-Harvey
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：November 4, 2015
- 年：2015
- 卷：63
- 期：43
- 页码：9471-9479
- 全文大小：350K
- ISSN：1520-5118
文摘
Little information exists on the effects of ensiling on condensed tannins or proanthocyanidins. The acetone鈥揵utanol鈥揌Cl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is of interest for deciphering the relationships between tannins and their bioactivities in terms of animal nutrition or health. Degradation with benzyl mercaptan (thiolysis) provides information on proanthocyanidin composition, but proanthocyanidins in several sainfoin silages have proved resistant to thiolysis. We now report that a pretreatment step with sodium hydroxide prior to thiolysis was needed to enable their analysis. This alkaline treatment increased their extractability from ensiled sainfoin and facilitated especially the release of larger proanthocyanidins. Ensiling reduced assayable proanthocyanidins by 29%, but the composition of the remaining proanthocyanidins in silage resembled that of the fresh plants.