Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase

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• 作者：Arnab Mukherjee ; Steven E. Rokita
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：December 16, 2015
• 年：2015
• 卷：137
• 期：49
• 页码：15342-15345
• 全文大小：285K
• ISSN：1520-5126

文摘

A single mutation within a flavoprotein is capable of switching the catalytic activity of a dehalogenase into a nitroreductase. This change in function correlates with a destabilization of the one-electron-reduced flavin semiquinone that is differentially expressed in the nitro-FMN reductase superfamily during redox cycling. The diversity of function within such a superfamily therefore has the potential to arise from rapid evolution, and its members should provide a convenient basis for developing new catalysts with an altered specificity of choice.