The Packaging of Different Cargo into Enveloped Viral Nanoparticles
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- 作者：Fan Cheng ; Irina B. Tsvetkova ; Y-Lan Khuong ; Alan W. Moore ; Randy J. Arnold ; Nancy L. Goicochea ; Bogdan Dragnea ; Suchetana Mukhopadhyay
- 刊名：Molecular Pharmaceutics
- 出版年：2013
- 出版时间：January 7, 2013
- 年：2013
- 卷：10
- 期：1
- 页码：51-58
- 全文大小：387K
- 年卷期：v.10,no.1(January 7, 2013)
- ISSN：1543-8392
文摘
Viral nanoparticles used for biomedical applications must be able to discriminate between tumor or virus-infected host cells and healthy host cells. In addition, viral nanoparticles must have the flexibility to incorporate a wide range of cargo, from inorganic metals to mRNAs to small molecules. Alphaviruses are a family of enveloped viruses for which some species are intrinsically capable of systemic tumor targeting. Alphavirus virus-like particles, or viral nanoparticles, can be generated from in vitro self-assembled core-like particles using nonviral nucleic acid. In this work, we expand on the types of cargo that can be incorporated into alphavirus core-like particles and the molecular requirements for packaging this cargo. We demonstrate that different core-like particle templates can be further enveloped to form viral nanoparticles that are capable of cell entry. We propose that alphaviruses can be selectively modified to create viral nanoparticles for biomedical applications and basic research.