Resonantly Enhanced Multiphoton Ionization Spectrum of the Neutral Green Fluorescent Protein Chromophore
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- 作者：Jason B. Greenwood ; Jordan Miles ; Simone De Camillis ; Peter Mulholland ; Lijuan Zhang ; Michael A. Parkes ; Helen C. Hailes ; Helen H. Fielding
- 刊名：The Journal of Physical Chemistry Letters
- 出版年：2014
- 出版时间：October 16, 2014
- 年：2014
- 卷：5
- 期：20
- 页码：3588-3592
- 全文大小：257K
- ISSN：1948-7185
文摘
The photophysics of the green fluorescent protein is governed by the electronic structure of the chromophore at the heart of its 尾-barrel protein structure. We present the first two-color, resonance-enhanced, multiphoton ionization spectrum of the isolated neutral chromophore in vacuo with supporting electronic structure calculations. We find the absorption maximum to be 3.65 卤 0.05 eV (340 卤 5 nm), which is blue-shifted by 0.5 eV (55 nm) from the absorption maximum of the protein in its neutral form. Our results show that interactions between the chromophore and the protein have a significant influence on the electronic structure of the neutral chromophore during photoabsorption and provide a benchmark for the rational design of novel chromophores as fluorescent markers or photomanipulators.