文摘
The oil from Madhuca indica has a high free fatty acid content, and hence, it is difficult to convert it intobiodiesel by using a chemical catalyst. In this work, a commercial preparation of lipase from Pseudomonascepacia was used as a catalyst for converting this oil into ethyl esters. Solvent-free media were used for thisprocess. The process optimization consisted of varying parameters, such as water content in the reaction mediumand salt present during the drying of lipase prior to its use as powder. The lipase immobilized on accurel gave96% conversion in 6 h. The best results were obtained using modified biocatalyst formulations, which arecalled cross-linked enzyme aggregates (CLEAs) and protein-coated microcrystals (PCMCs). While free enzymepowder after process optimization gave 98% conversion in 6 h using 50 mg of lipase, CLEAs gave 92%conversion in 2.5 h (using an equivalent of 50 mg of enzyme) and PCMCs gave 99% conversion in 2.5 husing the same amount of enzyme. In the case of PCMCs, 99% conversion could be obtained just by using abiocatalyst equivalent to 25 mg of enzyme but with an extended time period of 16 h.