Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform
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- 作者：Zhong Guo ; Lindy Murphy ; Viktor Stein ; Wayne A. Johnston ; Siro Alcala-Perez ; Kirill Alexandrov
- 刊名：Journal of the American Chemical Society
- 出版年：2016
- 出版时间：August 17, 2016
- 年：2016
- 卷：138
- 期：32
- 页码：10108-10111
- 全文大小：274K
- 年卷期：0
- ISSN：1520-5126
文摘
Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re-engineered component of glucometers: PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted for the detection of immunosuppressant drugs, α-amylase protein, or protease activity of thrombin and Factor Xa. The biosensors could be stored in dried form without appreciable loss of activity. We further show that ligand-induced activity of the developed biosensors could be directly monitored by chronoamperometry, enabling construction of disposable sensory electrodes. We expect that this architecture could be expanded to the detection of other biochemical activities, post-translational modifications, nucleic acids, and inorganic molecules.