Analysis of C-Reactive Protein on Amide-Linked N-Hydroxysuccinimide-Dextran Arrays with a Spectral Surface Plasmon Resonance Biosensor for Serodiagnosis

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• 作者：Se-Hui Jung ; Jae-Wan Jung ; In-Bum Suh ; Jong Seol Yuk ; Woo-Jin Kim ; Eui Yul Choi ; Young-Myeong Kim ; Kwon-Soo Ha
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：August 1, 2007
• 年：2007
• 卷：79
• 期：15
• 页码：5703 - 5710
• 全文大小：453K
• 年卷期：v.79,no.15(August 1, 2007)
• ISSN：1520-6882

文摘

A new label-free array system using amide-linked (AL)NHS-dextran and a spectral SPR biosensor are presentedfor the high-throughput analysis of C-reactive protein(CRP) in human sera. The AL NHS-dextran layer onthe surface of gold arrays was composed of an amidelinkage between NHS-modified carboxymethyl-dextranand amine-modified 11-mercaptoundecanoic acid. Thetopology of the AL NHS-dextran layer was analyzedby atomic force microscopy, and it was found to besuperior to the previously used epoxide-linked carboxymethyl-dextran layer in its immobilization of proteins.Specific immunoreactions and a dose-dependent increaseof SPR signals were demonstrated on the AL NHS-dextran layer. Then, the label-free array system wassuccessfully applied to the rapid analysis of CRP in 120human sera. CRP levels in human sera determined by thearray-based spectral SPR biosensor showed a good correlation with those determined by the latex-enhancedturbidimetry immunoassay (n = 120, r = 0.945, p <0.0001). Thus, the array-based spectral SPR biosensorbased on the AL NHS-dextran surface is a potentialsystem for rapid and label-free serodiagnosis of humandiseases.