文摘
Four peptides that inhibit angiotensin-converting enzyme (ACE) were separated from the hydorlysate of boneless chicken leg meat digested with artificial gastric juice (pepsin). Two peptides were identified as the peptides encrypted in myosin heavy chain. The peptide P1 (MNVKHWPWMK) corresponds to the amino acid sequence from amino acids 825 to 834 of myosin heavy chain, and the peptide P4 (VTVNPYKWLP) corresponds to the amino acid sequence from amino acids 125 to 135 of myosin heavy chain. They are novel ACE inhibitory peptides derived from chicken, and IC50 values of P1 and P4 were determined as 228 and 5.5 μM, respectively. Although these values were much larger than 0.022 μM for captopril, a typical synthetic ACE inhibitor, they are comparable to IC50 values reported for various ACE inhibitory peptides derived from foods. Because the peptide P4 has a relatively low IC50 value, it is a good starting substance for designing food supplements for hypertensive patients.