Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides
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- 作者：Shah Md. Asraful Islam ; Renukaradhya K. Math ; Kye Man Cho ; Woo Jin Lim ; Su Young Hong ; Jong Min Kim ; Myoung Geun Yun ; Ji Joong Cho ; Han Dae Yun
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：May 12, 2010
- 年：2010
- 卷：58
- 期：9
- 页码：5380-5386
- 全文大小：817K
- 年卷期：v.58,no.9(May 12, 2010)
- ISSN：1520-5118
文摘
Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 °C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion.