Two-Photon Lysotrackers for in Vivo Imaging

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• 作者：Ji Hoon Son ; Chang Su Lim ; Ji Hee Han ; Isravel Antony Danish ; Hwan Myung Kim ; Bong Rae Cho
• 刊名：Journal of Organic Chemistry
• 出版年：2011
• 出版时间：October 7, 2011
• 年：2011
• 卷：76
• 期：19
• 页码：8113-8116
• 全文大小：796K
• 年卷期：v.76,no.19(October 7, 2011)
• ISSN：1520-6904

文摘

We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750鈥?40 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time through two-photon microscopy. When combined with appropriate two-photon probes for other biological targets, these novel probes would greatly facilitate the two-photon microscopy colocalization experiments.