Chemical Synthesis of 23 kDa Glycoprotein by Repetitive Segment Condensation: A Synthesis of MUC2 Basal Motif Carrying Multiple O-GalNAc Moieties
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- 作者：Hironobu Hojo ; Yoshiyuki Matsumoto ; Yuko Nakahara ; Emi Ito ; Yusuke Suzuki ; Minoru Suzuki ; Akemi Suzuki ; and Yoshiaki Nakahara
- 刊名：Journal of the American Chemical Society
- 出版年：2005
- 出版时间：October 5, 2005
- 年：2005
- 卷：127
- 期：39
- 页码：13720 - 13725
- 全文大小：231K
- 年卷期：v.127,no.39(October 5, 2005)
- ISSN：1520-5126
文摘
Peptide thioester corresponding to a MUC2 tandem repeat unit, which retains seven GalNAcmoieties, was prepared by the Fmoc method followed by the low TfOH treatment to remove benzyl groupsat the carbohydrate portions. The glycosylated peptide thioester was then consecutively joined by theactivation of a thioester group by silver ions to obtain a MUC2 tandem repeat model composed of 141amino acids with 42 GalNAc moieties.