Carbohydrate−Protein Interactions Investigated on Plastic Chips Statically Coated with Hydrophobically Modified Hydroxyethylcellulose

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• 作者：Fuquan Dang ; Eiki Maeda ; Tomo Osafune ; Kazuki Nakajima ; Kazuaki Kakehi ; Mitsuru Ishikawa ; Yoshinobu Baba
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：December 15, 2009
• 年：2009
• 卷：81
• 期：24
• 页码：10055-10060
• 全文大小：237K
• 年卷期：v.81,no.24(December 15, 2009)
• ISSN：1520-6882

文摘

We developed a novel method for rapid screening of carbohydrate−protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from α1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.