Antimutagenic Activity of Gigantol from Dendrobium nobile
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- 作者：Mitsuo Miyazawa ; Hideo Shimamura ; Sei-ichi Nakamura ; and Hiromu Kameoka
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：1997
- 出版时间：August 1997
- 年：1997
- 卷：45
- 期：8
- 页码：2849 - 2853
- 全文大小：243K
- 年卷期：v.45,no.8(August 1997)
- ISSN：1520-5118
文摘
A methanol extract from Dendrobium nobile showed asuppressive effect on umu gene expressionof the SOS response in Salmonella typhimurium TA1535/pSK1002against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide). The methanolextract from D. nobile was re-extractedwith n-hexane, dichloromethane, 1-butanol, and water,respectively. A suppressive compound inthe n-hexane extract fraction was isolated bySiO2 column chromatography and chemicalfractionation and was identified as gigantol by EI-MS and1H and 13C NMR spectroscopy.Gigantolsuppressed the SOS-inducing activity of furylfuramide in theumu test. Gene expression wassuppressed 90% at <0.73 
mol/mL, and the ID50 value was0.35 mol/mL. Gigantol was also assayedwith the mutagen3-amino-1,4-dimethyl-5H-pyrido[4,3b]indole(Trp-P-1), which requires livermetabolizing enzymes, and it suppressed the SOS-inducing activity ofTrp-P-1 in the umu test.Gene expression was suppressed 91% at <0.73 mol/mL, and theID50 value was 0.32 mol/mL. Inaddition, gigantol was assayed for suppressive effect on UV irradiationin the umu test, where itshowed suppression of the SOS-inducing activity caused by UVirradiation. Gene expression wassuppressed 84% at <0.36 mol/mL, and the ID50 value was0.17 mol/mL. The antimutagenicactivities of gigantol against furylfuramide and Trp-P-1 were assayedby an Ames test using S.typhimurium TA100, which indicated that gigantol suppressed eachof the mutagens.Keywords: Dendrobium nobile; gigantol; Orchidaceae; antimutagenic activity;umu test; Ames test
mol/mL, and the ID50 value was0.35 mol/mL. Gigantol was also assayedwith the mutagen3-amino-1,4-dimethyl-5H-pyrido[4,3b]indole(Trp-P-1), which requires livermetabolizing enzymes, and it suppressed the SOS-inducing activity ofTrp-P-1 in the umu test.Gene expression was suppressed 91% at <0.73 mol/mL, and theID50 value was 0.32 mol/mL. Inaddition, gigantol was assayed for suppressive effect on UV irradiationin the umu test, where itshowed suppression of the SOS-inducing activity caused by UVirradiation. Gene expression wassuppressed 84% at <0.36 mol/mL, and the ID50 value was0.17 mol/mL. The antimutagenicactivities of gigantol against furylfuramide and Trp-P-1 were assayedby an Ames test using S.typhimurium TA100, which indicated that gigantol suppressed eachof the mutagens.Keywords: Dendrobium nobile; gigantol; Orchidaceae; antimutagenic activity;umu test; Ames test