Bacterial DNA Sample Preparation from Whole Blood Using Surface-Modified Si Pillar Arrays

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• 作者：Kyu-Youn Hwang ; Hee-Kyun Lim ; Seong-Young Jung ; Kak Namkoong ; Joon-Ho Kim ; Nam Huh ; Christopher Ko ; Jae-Chan Park
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：October 15, 2008
• 年：2008
• 卷：80
• 期：20
• 页码：7786-7791
• 全文大小：224K
• 年卷期：v.80,no.20(October 15, 2008)
• ISSN：1520-6882

文摘

A novel bacterial DNA sample preparation device for molecular diagnostics has been developed. On the basis of optimized conditions for bacterial adhesion, surface-modified silicon pillar arrays for bacterial cell capture were fabricated, and their ability to capture bacterial cells was demonstrated. The capture efficiency for bacterial cells such as Escherichia coli, Staphylococcus epidermidis, and Streptococcus mutans in buffer solution was over 75% with a flow rate of 400 μL/min. Moreover, the proposed method captured E. coli cells present in 50% whole blood effectively. The captured cells from whole blood were then in-situ lyzed on the surface of the microchip, and the eluted DNA was successfully amplified by qPCR. These results demonstrate that the full process of pathogen capture to DNA isolation from whole blood could be automated in a single microchip.