Ultrafine Cellulose Fibers Produced by Asaia bogorensis, an Acetic Acid Bacterium

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• 作者：Akio Kumagai ; Masahiro Mizuno ; Naoto Kato ; Kouichi Nozaki ; Eiji Togawa ; Shigeru Yamanaka ; Kazuo Okuda ; Inder M. Saxena ; Yoshihiko Amano
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：July 11, 2011
• 年：2011
• 卷：12
• 期：7
• 页码：2815-2821
• 全文大小：997K
• 年卷期：v.12,no.7(July 11, 2011)
• ISSN：1526-4602

文摘

The ability to synthesize cellulose by Asaia bogorensis, a member of the acetic acid bacteria, was studied in two substrains, AJ and JCM. Although both strains have identical 16S rDNA sequence, only the AJ strain formed a solid pellicle at the air鈥搇iquid interface in static culture medium, and we analyzed this pellicle using a variety of techniques. In the presence of cellulase, glucose and cellobiose were released from the pellicle suggesting that it is made of cellulose. Field emission electron microscopy allowed the visualization of a 3D knitted structure with ultrafine microfibrils (approximately 5鈥?0 nm in width) in cellulose from A. bogorensis compared with the 40鈥?00 nm wide microfibrils observed in cellulose isolated from Gluconacetobacter xylinus, suggesting differences in the mechanism of cellulose biosynthesis or organization of cellulose synthesizing sites in these two related bacterial species. Identifying these differences will lead to a better understanding of cellulose biosynthesis in bacteria.