Oenothera paradoxa Defatted Seeds Extract and Its Bioactive Component Penta-O-galloyl-β-d-glucose Decreased Production of Reactive Oxygen Species and
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- 作者：Anna K. Kiss ; Agnieszka Filipek ; Monika Czerwiska ; Marek Naruszewicz
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：September 22, 2010
- 年：2010
- 卷：58
- 期：18
- 页码：9960-9966
- 全文大小：860K
- 年卷期：v.58,no.18(September 22, 2010)
- ISSN：1520-5118
文摘
In this study, we analyzed ex vivo the effect of an aqueous extract of Oenothera paradoxa defatted seeds on the formation of neutrophil-derived oxidants. For defining active compounds, we also tested lypophilic extract constituents such as gallic acid, (+)-catechin, ellagic acid, and penta-O-galloyl-β-d-glucose and a hydrophilic fraction containing polymeric procyanidins. The anti-inflammatory potential of the extract and compounds was tested by determining the release from activated neutrophils of elastase, myeloperoxidase, interleukin-8 (IL-8), and leukotriene B4 (LTB4), which are considered relevant for the pathogenesis of cardiovascular diseases. The extract of O. paradoxa defatted seeds displays potent antioxidant effects against both 4β-phorbol-12β-myristate-α13-acetate- and formyl-met-leu-phenylalanine-induced reactive oxygen species production in neutrophils with IC50 values around 0.2 μg/mL. All types of polyphenolics present in the extract contributed to the extract antioxidant activity. According to their IC50 values, penta-O-galloyl-β-d-glucose was the more potent constituent of the extract. In cell-free assays, we demonstrated that this effect is partially due to the scavenging of O2− and H2O2 oxygen species. The extract and especially penta-O-galloyl-β-d-glucose significantly inhibit elastase, myeloperoxidase IL-8, and LTB4 release with an IC50 for penta-O-galloyl-β-d-glucose of 17 ± 1, 15 ± 1, 6.5 ± 2.5, and around 20 μM, respectively. The inhibition of penta-O-galloyl-β-d-glucose on reactive oxygen species and especially on O2− production, myeloperoxidase, and chemoattractant release may reduce the interaction of polymorphonuclear leukocyte with the vascular endothelium and by that potentially diminish the risk of progression of atherosclerosis development.