Pharmacokinetics of the Individual Major Components of Polymyxin B and Colistin in Rats

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• 作者：Sivashangarie Sivanesan ; Kade Roberts ; Jiping Wang ; Soon-Ee Chea ; Philip E. Thompson ; Jian Li ; Roger L. Nation ; Tony Velkov
• 刊名：Journal of Natural Products
• 出版年：2017
• 出版时间：January 27, 2017
• 年：2017
• 卷：80
• 期：1
• 页码：225-229
• 全文大小：362K
• ISSN：1520-6025

文摘

The pharmacokinetics of polymyxin B₁, polymyxin B₂, colistin A, and colistin B were investigated in a rat model following intravenous administration (0.8 mg/kg) of each individual component. Plasma and urine concentrations were determined by LC-MS/MS, and plasma protein binding was measured by ultracentrifugation. Total and unbound pharmacokinetic parameters for each component were calculated using noncompartmental analysis. All of the polymyxin components had a similar clearance, volume of distribution, elimination half-life, and urinary recovery. The area under the concentration–time curve for polymyxins B₁ and B₂ was greater than those of colistins A and B. Colistin A (56.6 ± 9.25%) and colistin B (41.7 ± 12.4%) displayed lower plasma protein binding in rat plasma compared to polymyxin B₁ (82.3 ± 4.30%) and polymyxin B₂ (68.4 ± 3.50%). These differences in plasma protein binding potentially equate to significant differences in unbound pharmacokinetics, highlighting the need for more stringent standardization of the composition of commercial products currently available for clinical use.