MitoBlue: A Nontoxic and Photostable Blue-Emitting Dye That Selectively Labels Functional Mitochondria

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• 作者：Mateo I. S谩nchez ; Jos茅 Mart铆nez-Costas ; Jos茅 L. Mascare帽as ; M. Eugenio V谩zquez
• 刊名：ACS Chemical Biology
• 出版年：2014
• 出版时间：December 19, 2014
• 年：2014
• 卷：9
• 期：12
• 页码：2742-2747
• 全文大小：305K
• ISSN：1554-8937

文摘

We report the discovery of a fluorogenic dye, N¹,N³-di(2-aminidonaphthalen-6-yl) propane-1,3-diamine, MitoBlue, which selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching. Additionally, we show that a biotin-labeled MitoBlue derivative can be used as a handle for the delivery of streptavidin-tagged species to the mitochondria.