Immunoassay To Quantify the Major Peach Allergen Pru p 3 in Foodstuffs. Differential Allergen Release and Stability under Physiological Conditions

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• 作者：Oscar A. Duffort ; Florentino Polo ; Manuel Lombardero ; Araceli Dí ; az-Perales ; Rosa Sá ; nchez-Monge ; Gloria Garcí ; a-Casado ; Gabriel Salcedo ; and Domingo Barber
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2002
• 出版时间：December 18, 2002
• 年：2002
• 卷：50
• 期：26
• 页码：7738 - 7741
• 全文大小：47K
• 年卷期：v.50,no.26(December 18, 2002)
• ISSN：1520-5118

文摘

Pru p 3 is a lipid transfer protein (LTP) that has been identified as the major peach (Prunus persica)allergen. However, little is known about the amount present in both raw and processed foodstuffs.Moreover, the in vivo release upon consumption of peach-containing foods remains unclear. Wehave developed a sensitive monoclonal antibody-based ELISA for Pru p 3. The method has beenapplied to measure the allergen levels in foodstuffs and the allergen release under differentphysiological conditions. A significant variability in all raw peaches and peach-containing foods testedhas been detected. The allergen was extracted more efficiently at a low pH, and it was highly resistantto pepsin. This ELISA will be very useful in controlling the allergen concentration in diagnostics, inevaluating threshold levels in provocation tests, and in detecting hidden allergens in processed foodsand cosmetics.Keywords: Peach; lipid transfer protein; allergen; food allergy; Prunus persica; Pru p 3; immunoassay;monoclonal antibody