Sensomics Mapping and Identification of the Key Bitter Metabolites in Gouda Cheese
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- 作者：Simone Toelstede ; Thomas Hofmann
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：April 23, 2008
- 年：2008
- 卷：56
- 期：8
- 页码：2795 - 2804
- 全文大小：568K
- 年卷期：v.56,no.8(April 23, 2008)
- ISSN：1520-5118
文摘
Application of a sensomics approach on the water-soluble extract of a matured Gouda cheese including gel permeation chromatography, ultrafiltration, solid phase extraction, preparative RP-HPLC, and HILIC combined with analytical sensory tools enabled the comprehensive mapping of bitter-tasting metabolites. LC-MS-TOF and LC-MS/MS, independent synthesis, and sensory analysis revealed the identification of a total of 16 bitter peptides formed by proteolysis of caseins. Eleven previously unreported bitter peptides were aligned to β-casein, among which 6 peptides were released from the sequence β-CN(57–69) of the N terminus of β-casein and 2 peptides originated from the C-terminal sequence β-CN(198–206). The other peptides were liberated from miscellaneous regions of β-casein, namely, β-CN(22–28), β-CN(74–86), β-CN(74–77), and β-CN(135–138), respectively. Six peptides were found to originate from αs1-casein and were shown to have the sequences αs1-CN(11–14), αs1-CN(56–60), αs1-CN(70/71–74), αs1-CN(110/111–114), and αs1-CN(135–136). Sensory evaluation of the purified, synthesized peptides revealed that 12 of these peptides showed pronounced bitter taste with recognition thresholds between 0.05 and 6.0 mmol/L. Among these peptides, the decapeptide YPFPGPIHNS exhibited a caffeine-like bitter taste quality at the lowest threshold concentration of 0.05 mmol/L.