Characterization of the Fe Site in Iron−Sulfur Cluster-Free Hydrogenase (Hmd) and of a Model Compound via Nuclear Resonance Vibrational Spectroscopy (NRVS)
详细信息 查看全文
- 作者：Yisong Guo ; Hongxin Wang ; Yuming Xiao ; Sonja Vogt ; Rudolf K. Thauer ; Seigo Shima ; Phillip I. Volkers ; Thomas B. Rauchfuss ; Vladimir Pelmenschikov ; David A. Case ; Ercan E. Alp ; Wolfgang Sturhahn ; Yoshi
- 刊名：Inorganic Chemistry
- 出版年：2008
- 出版时间：May 19, 2008
- 年：2008
- 卷：47
- 期：10
- 页码：3969 - 3977
- 全文大小：2387K
- 年卷期：v.47,no.10(May 19, 2008)
- ISSN：1520-510X
文摘
We have used 57Fe nuclear resonance vibrational spectroscopy (NRVS) to study the iron site in the iron−sulfur cluster-free hydrogenase Hmd from the methanogenic archaeon Methanothermobacter marburgensis. The spectra have been interpreted by comparison with a cis-(CO)2-ligated Fe model compound, Fe(S2C2H4)(CO)2(PMe3)2, as well as by normal mode simulations of plausible active site structures. For this model complex, normal mode analyses both from an optimized Urey–Bradley force field and from complementary density functional theory (DFT) calculations produced consistent results. For Hmd, previous IR spectroscopic studies found strong CO stretching modes at 1944 and 2011 cm−1, interpreted as evidence for cis-Fe(CO)2 ligation. The NRVS data provide further insight into the dynamics of the Fe site, revealing Fe−CO stretch and Fe−CO bend modes at 494, 562, 590, and 648 cm−1, consistent with the proposed cis-Fe(CO)2 ligation. The NRVS also reveals a band assigned to Fe−S stretching motion at ~311 cm−1 and another reproducible feature at ~380 cm−1. The 57Fe partial vibrational densities of states (PVDOS) for Hmd can be reasonably well simulated by a normal mode analysis based on a Urey–Bradley force field for a five-coordinate cis-(CO)2-ligated Fe site with additional cysteine, water, and pyridone cofactor ligands. A “truncated” model without a water ligand can also be used to match the NRVS data. A final interpretation of the Hmd NRVS data, including DFT analysis, awaits a three-dimensional structure for the active site.