Synthesis and Characterization of Polymer-Coated Quantum Dots with Integrated Acceptor Dyes as FRET-Based Nanoprobes

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• 作者：Marí ; a Teresa Ferná ; ndez-Arg&uuml ; elles ; Aleksey Yakovlev ; Ralph A. Sperling ; Camilla Luccardini ; Sté ; phane Gaillard ; Alfredo Sanz Medel ; Jean-Maurice Mallet ; Jean-Claude Brochon ; An
• 刊名：Nano Letters
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：7
• 期：9
• 页码：2613 - 2617
• 全文大小：94K
• 年卷期：v.7,no.9(September 2007)
• ISSN：1530-6992

文摘

A fluorescence resonance energy transfer pair consisting of a colloidal quantum dot donor and multiple organic fluorophores as acceptorsis reported and the photophysics of the system is characterized. Most nanoparticle-based biosensors reported so far use the detection ofspecific changes of the donor/acceptor distance under the influence of analyte binding. Our nanoparticle design on the other hand leads tosensors that detect spectral changes of the acceptor (under the influence of analyte binding) at fixed donor/acceptor distance by the introductionof the acceptor into the polymer coating. This approach allows for short acceptor-donor separation and thus for high-energy transfer efficiencies.Advantageously, the binding properties of the hydrophilic polymer coating further allows for addition of poly(ethylene glycol) shells for improvedcolloidal stability.