Distinguishing Monosaccharide Stereo- and Structural Isomers with TOF-SIMS and Multivariate Statistical Analysis

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• 作者：Elena S. F. Berman ; Kristen S. Kulp ; Mark G. Knize ; Ligang Wu ; Erik J. Nelson ; David O. Nelson ; Kuang Jen Wu
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：September 15, 2006
• 年：2006
• 卷：78
• 期：18
• 页码：6497 - 6503
• 全文大小：181K
• 年卷期：v.78,no.18(September 15, 2006)
• ISSN：1520-6882

文摘

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is utilized to examine the mass spectra andfragmentation patterns of seven isomeric monosaccharides. Multivariate statistical analysis techniques, including principal component analysis (PCA), allow discrimination of the extremely similar mass spectra of stereoisomers. Furthermore, PCA identifies those fragmentpeaks that vary significantly between spectra. Heavyisotope studies confirm that these peaks are indeed sugarfragments, allow identification of the fragments, andprovide clues to the fragmentation pathways. Excellentreproducibility is shown by multiple experiments performed over time and on separate samples. This studydemonstrates the combined selectivity and discriminationpower of TOF-SIMS and PCA and suggests new applications of the technique including differentiation of subtlechemical changes in biological samples that may provideinsights into cellular processes, disease progress, anddisease diagnosis.