Proteomic Analysis of Pancreatic Zymogen Granules: Identification of New Granule Proteins

详细信息    查看全文

• 作者：Michael J. Rindler ; Chong-feng Xu ; Iwona Gumper ; Nora N. Smith ; Thomas A. Neubert
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：August 2007
• 年：2007
• 卷：6
• 期：8
• 页码：2978 - 2992
• 全文大小：662K
• 年卷期：v.6,no.8(August 2007)
• ISSN：1535-3907

文摘

The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enrichedintragranular content, peripheral membrane, and integral membrane protein fractions were analyzedafter one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identifiedwith high confidence, including 84 previously identified granule proteins. The 287 remaining proteinsincluded 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels andtransporters. Seven proteins, pantophysin, cyclic nucleotide phosphodiesterase, carboxypeptidase D,ecto-nucleotide phosphodiesterase 3, aminopeptidase N, ral, and the potassium channel TWIK-2, wereconfirmed by immunofluorescence microscopy or by immunoblotting to be new zymogen granulemembrane proteins.