Screening for EphB Signaling Effectors Using SILAC with a Linear Ion Trap-Orbitrap Mass Spectrometer

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• 作者：Guoan Zhang ; David Fenyö ; Thomas A. Neubert
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：November 7, 2008
• 年：2008
• 卷：7
• 期：11
• 页码：4715-4726
• 全文大小：331K
• 年卷期：v.7,no.11(November 7, 2008)
• ISSN：1535-3907

文摘

Erythropoietin-producing hepatocellular carcinoma (Eph) receptors play important roles in development, neural plasticity, and cancer. We used an Orbitrap mass spectrometer and stable isotope labeling by amino acids in cell culture (SILAC) to identify and quantify 204 proteins with significantly changed abundance in antiphosphotyrosine immunoprecipitates after ephrinB1-Fc stimulation. More than half of all known effectors downstream of EphB receptors were identified in this study, as well as numerous novel candidates for EphB signaling.