Detection of Nitric Oxide Release from Single Neurons in the Pond Snail, Lymnaea stagnalis
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- 作者：Bhavik Anil Patel ; Martin Arundell ; Kim H. Parker ; Mark S. Yeoman ; Danny O'Hare
- 刊名：Analytical Chemistry
- 出版年：2006
- 出版时间：November 15, 2006
- 年：2006
- 卷：78
- 期：22
- 页码：7643 - 7648
- 全文大小：197K
- 年卷期：v.78,no.22(November 15, 2006)
- ISSN：1520-6882
文摘
Multiple film-coated nitric oxide sensors have been fabricated using Nafion and electropolymerized polyeugenolor o-phenylenediamine on 30-
m carbon fiber disk electrodes. This is a rare study that utilizes disk electrodesrather than the widely used protruding tip microelectrodesin order to measure from a biological environment. Theseelectrodes have been used to evaluate the differences innitric oxide release between two different identified neurons in the pond snail, Lymnaea stagnalis. These resultsshow the first direct measurements of nitric oxide releasefrom individual neurons. The electrodes are very sensitiveto nitric oxide with a detection limit of 2.8 nM and asensitivity of 9.46 nA M-1. The sensor was very selectiveagainst a variety of neurochemical interferences such asascorbic acid, uric acid, and catecholamines and secondary oxidation products such as nitrite. Nitric oxide releasewas measured from the cell bodies of two neurons, thecerebral giant cell (CGC) and the B2 buccal motor neuron,in the intact but isolated CNS. A high-Ca2+/high-K+stimulus was capable of evoking reproducible release. Fora given stimulus, the B2 neuron released more nitricoxide than the CGC neuron; however, both cells wereequally suppressed by the NOS inhibitor L-NAME.
m carbon fiber disk electrodes. This is a rare study that utilizes disk electrodesrather than the widely used protruding tip microelectrodesin order to measure from a biological environment. Theseelectrodes have been used to evaluate the differences innitric oxide release between two different identified neurons in the pond snail, Lymnaea stagnalis. These resultsshow the first direct measurements of nitric oxide releasefrom individual neurons. The electrodes are very sensitiveto nitric oxide with a detection limit of 2.8 nM and asensitivity of 9.46 nA M-1. The sensor was very selectiveagainst a variety of neurochemical interferences such asascorbic acid, uric acid, and catecholamines and secondary oxidation products such as nitrite. Nitric oxide releasewas measured from the cell bodies of two neurons, thecerebral giant cell (CGC) and the B2 buccal motor neuron,in the intact but isolated CNS. A high-Ca2+/high-K+stimulus was capable of evoking reproducible release. Fora given stimulus, the B2 neuron released more nitricoxide than the CGC neuron; however, both cells wereequally suppressed by the NOS inhibitor L-NAME.