Simplified 2-Aminoquinoline-Based Scaffold for Potent and Selective Neuronal Nitric Oxide Synthase Inhibition
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- 作者：Maris A. Cinelli ; Huiying Li ; Georges Chreifi ; Pavel Mart谩sek ; Linda J. Roman ; Thomas L. Poulos ; Richard B. Silverman
- 刊名：Journal of Medicinal Chemistry
- 出版年：2014
- 出版时间：February 27, 2014
- 年：2014
- 卷：57
- 期：4
- 页码：1513-1530
- 全文大小：736K
- 年卷期：v.57,no.4(February 27, 2014)
- ISSN：1520-4804
文摘
Since high levels of nitric oxide (NO) are implicated in neurodegenerative disorders, inhibition of the neuronal isoform of nitric oxide synthase (nNOS) and reduction of NO levels are therapeutically desirable. Nonetheless, many nNOS inhibitors mimic l-arginine and are poorly bioavailable. 2-Aminoquinoline-based scaffolds were designed with the hope that they could (a) mimic aminopyridines as potent, isoform-selective arginine isosteres and (b) possess chemical properties more conducive to oral bioavailability and CNS penetration. A series of these compounds was synthesized and assayed against purified nNOS enzymes, endothelial NOS (eNOS), and inducible NOS (iNOS). Several compounds built on a 7-substituted 2-aminoquinoline core are potent and isoform-selective; X-ray crystallography indicates that aminoquinolines exert inhibitory effects by mimicking substrate interactions with the conserved active site glutamate residue. The most potent and selective compounds, 7 and 15, were tested in a Caco-2 assay and showed good permeability and low efflux, suggesting high potential for oral bioavailability.