Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase

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• 作者：Huiying Li ; Heng-Yen Wang ; Soosung Kang ; Richard B. Silverman ; Thomas L. Poulos
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：July 5, 2016
• 年：2016
• 卷：55
• 期：26
• 页码：3702-3707
• 全文大小：435K
• 年卷期：0
• ISSN：1520-4995

文摘

Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the structural basis for why some dipeptide amide inhibitors bind more tightly to nNOS than to eNOS. We now have found that the same amino acid variation is responsible for substantially different binding modes and affinity for a new class of aminopyridine-based inhibitors.