Efficient Pro-survival/angiogenic miRNA Delivery by an MRI-Detectable Nanomaterial
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- 作者：Renata S. M. Gomes ; Ricardo Pires das Neves ; Lowri Cochlin ; Ana Lima ; Rui Carvalho ; Petra Korpisalo ; Galina Dragneva ; Mikko Turunen ; Timmo Liimatainen ; Kieran Clarke ; Seppo Yl盲-Herttuala ; Carolyn Carr ; Lino Ferreira
- 刊名：ACS Nano
- 出版年：2013
- 出版时间：April 23, 2013
- 年：2013
- 卷：7
- 期：4
- 页码：3362-3372
- 全文大小：701K
- 年卷期：v.7,no.4(April 23, 2013)
- ISSN：1936-086X
文摘
Herein, we report the use of biodegradable nanoparticles (NPs) containing perfluoro-1,5-crown ether (PFCE), a fluorine-based compound (NP170-PFCE) with the capacity to track cells in vivo by magnetic ressonance imaging (MRI) and efficiently release miRNA. NP170-PFCE complexed with miRNAs accumulate whitin the cell鈥檚 endolysosomal compartment and interact with higher frequency with argonaute2 (Ago2) and GW182 proteins, which are involved in the biological action of miRNAs, than commercial complexes formed by commercial reagents and miRNA, which in turn accumulate in the cell cytoplasm. The release of miRNA132 (miR132) from the NPs increased 3-fold the survival of endothelial cells (ECs) transplanted in vivo and 3.5-fold the blood perfusion in ischemic limbs relatively to control.