Isotope-Coded Labeling for Accelerated Protein Interaction Profiling Using MS

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• 作者：John D. Venable ; Caitlin Steckler ; Weijia Ou ; Jan Gr眉newald ; Sanjay Agarwalla ; Ansgar Brock
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：August 4, 2015
• 年：2015
• 卷：87
• 期：15
• 页码：7540-7544
• 全文大小：263K
• ISSN：1520-6882

文摘

Protein interaction surface mapping using MS is widely applied but comparatively resource-intensive. Here, a workflow adaptation for use of isotope-coded tandem mass tags for the purpose is reported. The key benefit of improved throughput derived from sample acquisition multiplexing and automated analysis is shown to be maintained in the new application. Mapping of the epitopes of two monoclonal antibodies on their respective targets serves to illustrate the novel approach. We conclude that the approach enables mapping of interactions by MS at significantly larger scales than hereto possible.