Sensing Technique of Silver Nanoparticles as Labels for Immunoassay Using Liquid Electrode Plasma Atomic Emission Spectrometry
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- 作者：Nguyen Hoang Tung ; Miyuki Chikae ; Yoshiaki Ukita ; Pham Hung Viet ; Yuzuru Takamura
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：February 7, 2012
- 年：2012
- 卷：84
- 期：3
- 页码：1210-1213
- 全文大小：220K
- 年卷期：v.84,no.3(February 7, 2012)
- ISSN：1520-6882
文摘
We report the use of liquid electrode plasma-atomic emission spectrometry (LEP-AES) in protein sensing studies employing Ag nanoparticle labeling. LEP-AES requires no plasma gas and no high-power source and is suitable for onsite portable analysis. Human chorionic gonadotropin (hCG) was used as a model target protein, and the immunoreaction in which hCG is sandwiched between two antibodies, one of which is immobilized on the microwell and the second is labeled with Ag nanoparticles, was performed. Sensing occurs at the narrow pass in the center of a quartz chip following oxidative dissolution of the Ag nanoparticles by nitric acid. hCG was analyzed in the range from 10 pg/mL to 1 ng/mL, and the detection limit for hCG was estimated at 1.3 pg/mL (22.8 fM). The proposed detection method has a wide variety of promising applications in metal-nanoparticle-labeled biomolecule detection.